Answer each question to the best of your ability. Show ALL calculations and use complete sentences. One-‐
word answers will never be given credit.
1. Do you expect your cells transformed with PRS316 to be blue or white? Do you expect your cells
transformed with the mystery plasmid to be blue or white? Explain. (2 points)
2. Explain the role of each of these reagents in next week’s experiment (1 point each):
a. Lactose
b. Toluene
c. ONPG
d. NaCO3
3. The cells we are transforming lack LacZ and its promoter in the bacterial genome. What do you expect would happen if we transformed LacZ+ cells with your plasmid? Why would it be a problem? (2 points)
4. In testing this lab, Biology 411 students found no difference in the amount of LacZ expression when lactose was added compared to samples where no sugar supplement was added. Describe a change to the plasmid sequence that would mean that all cells fail to respond to lactose (2 points).
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