The Effect of low pH on Enzyme Activity in Raw Meat
Purpose: To recognize the impact of acid on based on the activity of enzymes
Hypothesis: When acetic fluids are added to enzyme-substrate mixtures, enzyme activity is affected.
Experiment: Observation of the yeast and temperature regulated hydrogen peroxide in the presence of food.
Materials:
• Name of enzyme you will use: Yeast
• Name of organism (if applicable): Liver, carrot
• The substrate: Hydrogen peroxide
• Method for measuring enzyme activity: Circumference of balloons
• Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples: length of exposure will be 1 min, 3 min, 5 min
• The control(s) in the experiment: 3 Tubes filled with 10ml of catalase, 10ml of hydrogen peroxide and no acetic acid
• Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen peroxide and each one with 1ml, 3ml, 5ml of acetic acid
• Hypothesis: When acetic fluids are added to enzyme-substrate mixtures, enzyme activity is affected.
• How you will present your data (table and/or type of graph): Table
Procedure: 1. Cut 1 cm of meet and add to 200 ml cylinder
2. Add yeast into the same 200 ml cylinder
3. Add Hydrogen peroxide into the testing tube.
4. Cylinder and testing tube is kept in water both temperatures 80 degrees F.
5. Pour hot Hydrogen Peroxide in the cylinder that contains meat and yeast.
6. Volume of both cylinder and testing tube will be measured, before and after combining all items.
7. After 3 minutes the volume is measured again and recorded
8. Initial volume will be subtracted from final volume, to obtain the actual amount of foam.
9. Repeat by replace the acidic food with the alkaline one.
10. The data obtained will be used to create the table.
Data and Analysis: observing the altered pH condition and the level of oxygen that is generated will collect Data
Conclusion: the results of my exeriment
References: Any references used (i.e.: internet, books, documentation)
Use the order calculator below and get started! Contact our live support team for any assistance or inquiry.
[order_calculator]