Hypothesis: Depletion of RANKL decreases pulmonary M cell differentiation and protects against prion infection by the inhaled route.
1. Summary of proposed research ( 400 words)
2. Aims: 1. Depletion of M cells in the FAE of Peyer’s patches by RANKL neutralization. 2. M cell-depletion blocks prion neuroinvasion from the inhaled route
3. Background: Provide figures and data, review of previous research in the area and justification for further research.
4. Experimental design (with table) and methods, expected outcomes and their importance
• Animals: C57BL/6, Edinburgh, treatment with anti-RANKL, anti-IgG(not specific) as a control.
• Inoculation and tissue collection: Prion exposure by an inhalation chambers containing a nebulizer device.
• Disease monitoring: Behavior test ( memory and motor tests)
• Histological analysis: Immunohistochemistry and immunoflorescent
5. Prevention of Bias: Blinding, Randomization etc…
6. Data Analysis
7. Future Direction
8. Justify staff, materials and consumables requested providing estimates of total cost
9. Timetable plan
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